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Skin Microbiome Testing As the skincare industry grows, consumers demand transparency from the brands formulating their products. It has become essential for formulators to create products they are willing to test to present scientifically backed data-driven evidence of their products' true effects. Sequential offers microbiome testing for your skincare formulations, ranging from moisturizers, serums, cleansers, SPF products, etc. We are dedicated to understanding how your product interacts with the skin and its microbes. Depending on how in-depth you want to go, we offer qPCR, 16S, ITS, and Shotgun Metagenomics. Download Case Study! Personalized Approach to Testing Unlike other methodologies present within the industry, Sequential's approach ensures that your product's data and analysis will stand the test of industry regulations when they are introduced. You can tailor your study entirely to your unique requirements. Test Products in a Real-Life Context The microbiome comprises a complex ecosystem of microorganisms that live together in a delicate balance, which is why it's best to test directly upon it directly. To fully understand the impact a product is having on the microbiome, in vivo is the only way. Collect Longitudinal Data With in vivo testing we can design your study around the extended use of a product over multiple time points. This allows us to review how a product is performing before and after usage, but also take into account its gradual impact on the microbiome. Measure Against a Control Group Measure a product against a control group that might have a different percentage of your active ingredient within its formulation or no active at all. This will allow for deeper insights into the impact of a formulation on microbial balance and diversity. 4 Sequencing Reports To Pick From Depending on your development stage and what you are interested in studying we offer qPCR, 16S, ITS, and Shotgun Metagenomics. With our qPCR Smart Probes™ we can go down to the strain level in our analysis. Personalize Your Microbiome Study! The body's skin surface consists of the largest organ within the human body, hosting trillions of microbes (bacteria, viruses, fungi, etc.). These microbes protect the body from external forces, playing a key role in systemic human health. Clinically Validated Collection System In addition to skin swabs, at Sequential we have developed another strong innovation to collect reliable, reproducible, and straight forward skin samples - making it that much easier to do remote consumer and clinical studies. - Validated with external parties for DNA and RNA collection, both from microbial and host cells. - Published in peer-reviewed journals, showing ease of collection and adoption for clinical studies in a range of studies. -Clinically tested on 7 unique body sites. Smart Probe s ™ Our dedicated team of scientists have developed a method of evaluating microbes through our Smart Probes™. These refer to a panel of 20 key microbes we have specifically identified as having the most impact on skin health. Over and above the taxonomic characterisation that 16S offers, which gives us a snapshot of all the genus present within a collected sample (Cutibacterium, Staphyloccocus, etc.) our targeted approach takes it a step further, opening the lens to the species (C. acnes ), sub-species (C. acnes defendens ) and even strains within them. This is a crucial distinction as not all strains of a species behave similarly. In Fact, we find that within these species there are strains associated with inflammation and strains that are commensal, and beneficial. Gold Standard Certification Sequential has developed the gold standard test for products designed to target the microbiome, in vivo (in, or on, humans). Finally, we can give some certainty about if a product is truly affecting the microbiome. Using next-generation sequencing of the collection of micro-organisms found on the body, before and after product usage, Sequential investigates the microbial balance and diversity, and particular micro-organisms we know are important and play a role in a healthy microbiome. We give you an in vivo certification that your product maintains the microbiome. And it’s not exclusive to skincare! We do this for haircare products, oral products, and vulva/vaginal microbiomes. Personalize Your Microbiome Study! Supplement Your Microbiome Study Recruitment Services Let us take care of the entire candidate recruitment process for you! View More Biophysical Assessments Increase your data on the use of your product by evaluating additional biophysical factors. View More Formulation Support Seek consultation advice for your formulation if you are re-formulating or developing a new product. View More FAQ What is Sequential's testing platform? Sequential has developed the gold standard test for microbiome-friendly products, in vivo (in, or on, humans). Finally, we can give some certainty about if a product is truly affecting the microbiome. We offer a complete end-to-end solution to support microbiome-friendly claims. From consultancy and study design to our proprietary microbiome testing kits. We analyse, interpret and report our findings to meet your needs. Why is it necessary to test the microbiome in vivo? At present, there are no regulations for microbiome-related formulas that brands and formulators can follow, however, it has been universally acknowledged that the in vivo method of conducting clinical studies is becoming critical and paramount to getting marketing claims through. When regulations are introduced, which may be imminent, the in vitro system will find itself lacking, resulting in limited claims and certifications that do not hold their value. This is why, we at Sequential strive to offer an in vivo approach, knowing full well that we want our client's claims to be significantly backed by scientific and quantifiable data. What type of sequencing technology does Sequential use for analysis? We offer four types of sequencing techniques including qPCR with our Smart Probes™, 16S, ITS and Shotgun Metagenomics. Using next-generation sequencing of the collection of microorganisms found on the body, during product usage, Sequential investigates the microbial diversity, and particular microorganisms we know are important and play a role in a healthy microbiome. Does Sequential offer claims certification for tested products? We provide our clients with a certification to claim “Maintains the Microbiome” subject to in vivo testing results which can be used in communication efforts. Once your product is tested with our qPCR Smart Probes™ and has shown favourable results in supporting the microbiome, we can certify your product with our Maintains the Microbiome certification seal. We have ensured that our seal and certification are backed by quantifiable data and scientifically significant markers. The aim is to ensure our clients feel confident in making their claims and can communicate the true benefit of their microbiome formulations.

Clinical Assessments for More In-Depth Analysis When it comes to testing your personal care products, we offer add-ons to supplement your microbiome research efforts and facilitate your testing experience. It has become clear in recent years that differentiation is key when creating a formulation for the competitive market, however, differentiation can be incredibly straightforward when backed with the data and scientific evidence to support it. Not only will you differentiate your brand, you will also demonstrate your commitment to providing leading-edge and effective solutions for your customers’ personal care and skincare needs. Start Your Biophysical Study Today! The only way to understand how a product performs is by putting it to the test Hydration Measurement (Corneometry and TEWL) Gain a comprehensive understanding of your product's impact on moisture retention, skin hydration and overall skin barrier function. This data can guide the development of effective moisturizing formulations. Perception Questionnaire Uncovering how users experience and interact with products. These surveys offer valuable insights into subjective factors like texture, scent, and overall satisfaction ensuring that your scientific data is enriched. Elasticity Measurement (Elastometer) Unravel the effect your product has on the skin’s elasticity through the use of elastometers, allowing you to position your product within a firming category with demonstrated benefits for graceful aging. High-Resolution Imaging Use before and after images to provide visual evidence of a product’s impact on the skin’s appearance. These tangible comparisons offer brands the ability to objectively assess changes in the skin. Dermatologist Assessment Measure and quantify inflammatory disease, atopic dermatitis (through SCORAD), psoriasis (through PASI), comedogenicity, and other hallmarks of skin conditions, by our board-certified and in-house dermatologists. Skin pH Measurement Maintain the delicate balance of the skin's acid mantle with your product by measuring pH to ensure compatibility and minimise the risk of irritation for a gentle, biome supporting formulation. FAQ What is Sequential's testing platform? Sequential has developed the gold standard test for microbiome-friendly products, in vivo (in, or on, humans). Finally, we can give some certainty about if a product is truly affecting the microbiome. We offer a complete end-to-end solution to support microbiome-friendly claims. From consultancy and study design to our proprietary microbiome testing kits. We analyse, interpret and report our findings to meet your needs. Why is it necessary to test the microbiome in vivo? At present, there are no regulations for microbiome-related formulas that brands and formulators can follow, however, it has been universally acknowledged that the in vivo method of conducting clinical studies is becoming critical and paramount to getting marketing claims through. When regulations are introduced, which may be imminent, the in vitro system will find itself lacking, resulting in limited claims and certifications that do not hold their value. This is why, we at Sequential strive to offer an in vivo approach, knowing full well that we want our client's claims to be significantly backed by scientific and quantifiable data. What type of sequencing technology does Sequential use for analysis? We offer four types of sequencing techniques including qPCR with our Smart Probes™, 16S, ITS and Shotgun Metagenomics. Using next-generation sequencing of the collection of microorganisms found on the body, during product usage, Sequential investigates the microbial diversity, and particular microorganisms we know are important and play a role in a healthy microbiome. Does Sequential offer claims certification for tested products? We provide our clients with a certification to claim “Maintains the Microbiome” subject to in vivo testing results which can be used in communication efforts. Once your product is tested with our qPCR Smart Probes™ and has shown favourable results in supporting the microbiome, we can certify your product with our Maintains the Microbiome certification seal. We have ensured that our seal and certification are backed by quantifiable data and scientifically significant markers. The aim is to ensure our clients feel confident in making their claims and can communicate the true benefit of their microbiome formulations.

TARGETED SKIN PANEL Atopic Dermatitis Targeted Panel 23 Smart Probes ™ Atopic Dermatitis Panel Validated targets: Staphylococcus aureus, Malassezia spp, Staphylococcus epidermidis Absolute Quantification End-to-end Service Qet a Quote Atopic Dermatitis Study with Sequential's Cutting-Edge Microbiome Testing Why Microbiome Testing for Atopic Dermatitis? Atopic dermatitis (AD), a chronic inflammatory skin condition, affects around 31.6 million people in the U.S., with 15-20% of children globally and 7.3% of U.S. adults experiencing the condition (National Eczema Association). AD often results from a combination of genetic predisposition and environmental triggers, such as irritants in cosmetic products. Disruptions to the skin’s microbiome play a significant role in the disease's development and severity. Imbalances in beneficial and harmful microbes can exacerbate AD, highlighting the importance of targeted treatments. In vivo testing for products aimed at managing AD is essential because it allows researchers to understand how formulations impact both the skin barrier and microbiome in real-world conditions. Properly tested products can help reduce irritation and avoid worsening symptoms, offering a science-backed approach to skincare for those with AD. Sequential is leading the way in microbiome testing for the industry. With a specialized focus on skin conditions, we have developed an innovative solution tailored to help you understand the root causes of AD at the microbial level. Our proprietary sequencing panel is designed specifically to offer in-depth analysis of AD-associated microbiota, giving you the tools to transform skincare solutions. Customized Analysis Tailored insights specific to AD-prone skin, allowing your R&D team to develop more personalized and effective products. Actionable Data Leverage detailed reports to guide formulation decisions and validate product efficacy with precision. Product Innovation New AD treatments or enhancing existing lines, our data helps you create targeted solutions with proven results. Precision Sequencing Target specific microbial communities associated with AD development and severity. Smart Probe s ™ Our dedicated team of scientists has developed a revolutionary method for evaluating microbes through our Smart Probes™. This proprietary panel includes 23 key microbes that we have specifically identified as having the most significant impact on skin health. To further understand the intricate relationship between atopic dermatitis (AD) and the skin microbiome, we use qPCR technology. This advanced technique allows us to precisely quantify microbial populations on the skin, providing deeper insights into how AD affects the microbiome. By closely monitoring how products interact with both beneficial and harmful microbes, companies can create solutions for AD but also restore a healthy balance to the skin’s ecosystem. Atopic Dermatitis Panel Targets Staphylococcus aureus This bacterium is frequently found in higher numbers on the skin of individuals with atopic dermatitis. It can exacerbate inflammation and contribute to skin barrier disruption. Malassezia spp This genus of yeast, which is part of the normal skin flora, can become overgrown in individuals with atopic dermatitis, potentially aggravating symptoms. Staphylococcus epidermidis While generally considered a commensal organism, imbalances in its population, along with other microbes, can influence the skin's health and contribute to atopic dermatitis symptoms. Industry Leader in Absolute Quantification Sequential’s Smart Probe ™ qPCR approach to microbiome analysis can resolve species and subspecies, giving statistically significant findings between skin conditions on in vivo human samples, compared to conventional sequencing approaches. Relevant Research AOB for AD: Ammonium-oxidising Bacteria as an Innovative Approach to Managing Atopic Dermatitis Answers for Atopic Dermatitis and Allergies: What is the Role of the Skin Bacteriome? Understanding Atopic Dermatitis Read More Articles

Scalp Microbiome Testing As the scalp care industry grows, consumers demand transparency from the brands formulating their products. It has become essential for formulators to create products they are willing to test to present scientifically backed data-driven evidence of their products' true effects. Sequential offers microbiome testing for your scalp care formulations, ranging from scalp serums, shampoos, conditioners, oils, etc. We are dedicated to understanding how your product interacts with the scalp and its microbes. Depending on how in-depth you want to go, we offer qPCR, 16S, ITS, and Shotgun Metagenomics. Download Case Study! Personalized Approach to Testing Unlike other methodologies present within the industry, Sequential's approach ensures that your product's data and analysis will stand the test of industry regulations when they are introduced. You can tailor your study entirely to your unique requirements. Test Products in a Real-Life Context The microbiome comprises a complex ecosystem of microorganisms that live together in a delicate balance, which is why it's best to test directly upon it directly. To fully understand the impact a product is having on the microbiome, in vivo is the only way. Collect Longitudinal Data With in vivo testing we can design your study around the extended use of a product over multiple time points. This allows us to review how a product is performing before and after usage, but also take into account its gradual impact on the microbiome. Measure Against a Control Group Measure a product against a control group that might have a different percentage of your active ingredient within its formulation or no active at all. This will allow for deeper insights into the impact of a formulation on microbial balance and diversity. 4 Sequencing Reports To Pick From Depending on your development stage and what you are interested in studying we offer qPCR, 16S, ITS, and Shotgun Metagenomics. With our qPCR Smart Probes™ we can go down to the strain level in our analysis. Personalize Your Microbiome Study! Unlike hair, the scalp is formed of a living community of microorganisms such as bacterial and fungal players that can influence the balance of the scalp microbiome, even the subtlest of imbalances can lead to issues such as dandruff, itching, and irritation, resulting in unwanted flaking. Smart Probe s ™ Our dedicated team of scientists have developed a method of evaluating microbes through our Smart Probes ™ . These refer to a panel of 20 key microbes we have specifically identified as having the most impact on scalp health. Over and above the taxonomic characterisation that 16S offers, which gives us a snapshot of all the genus present within a collected sample (Cutibacterium , Staphylococcus , etc.) our targeted approach takes it a step further, opening the lens to the species (C. acnes ), sub-species (C. acnes defendens ) and even strains within them. This is a crucial distinction as not all strains of a species behave similarly. We find that within these species there are strains associated with inflammation and strains that are commensal, and beneficial. Gold Standard Certification Sequential has developed the gold standard test for products designed to target the microbiome, in vivo (in, or on, humans). Finally, we can give some certainty about if a product is truly affecting the microbiome. Using next-generation sequencing of the collection of micro-organisms found on the body, before and after product usage, Sequential investigates the microbial balance and diversity, and particular micro-organisms we know are important and play a role in a healthy microbiome. We give you an in vivo certification that your product maintains the microbiome. And it’s not exclusive to skincare! We do this for haircare products, oral products, and vulva/vaginal microbiomes. Personalize Your Microbiome Study! Supplement Your Microbiome Study Recruitment Services Let us take care of the entire candidate recruitment process for you! View More Biophysical Assessments Increase your data on the use of your product by evaluating additional biophysical factors. View More Formulation Support Seek consultation advice for your formulation if you are re-formulating or developing a new product. View More FAQ What is Sequential's testing platform? Sequential has developed the gold standard test for microbiome-friendly products, in vivo (in, or on, humans). Finally, we can give some certainty about if a product is truly affecting the microbiome. We offer a complete end-to-end solution to support microbiome-friendly claims. From consultancy and study design to our proprietary microbiome testing kits. We analyse, interpret and report our findings to meet your needs. Why is it necessary to test the microbiome in vivo? At present, there are no regulations for microbiome-related formulas that brands and formulators can follow, however, it has been universally acknowledged that the in vivo method of conducting clinical studies is becoming critical and paramount to getting marketing claims through. When regulations are introduced, which may be imminent, the in vitro system will find itself lacking, resulting in limited claims and certifications that do not hold their value. This is why, we at Sequential strive to offer an in vivo approach, knowing full well that we want our client's claims to be significantly backed by scientific and quantifiable data. What type of sequencing technology does Sequential use for analysis? We offer four types of sequencing techniques including qPCR with our Smart Probes™, 16S, ITS and Shotgun Metagenomics. Using next-generation sequencing of the collection of microorganisms found on the body, during product usage, Sequential investigates the microbial diversity, and particular microorganisms we know are important and play a role in a healthy microbiome. Does Sequential offer claims certification for tested products? We provide our clients with a certification to claim “Maintains the Microbiome” subject to in vivo testing results which can be used in communication efforts. Once your product is tested with our qPCR Smart Probes™ and has shown favourable results in supporting the microbiome, we can certify your product with our Maintains the Microbiome certification seal. We have ensured that our seal and certification are backed by quantifiable data and scientifically significant markers. The aim is to ensure our clients feel confident in making their claims and can communicate the true benefit of their microbiome formulations.

The skin health tracker Benefit of your personal Skin Health Tracker Download your personal Skin Health Tracker app to receive your microbiome test results and unlock personalized skincare tips. Results Skin Profile Receive your comprehensive skin microbiome sample results. Results Accessibility Access to your Skin Profile results in the palm of your hand. Results Unlock Our Expertise Learn skincare tips to help you with your own perceived skin traits. Results Discover Learn more about the skin microbiome through our Discover articles. What is Sequential's testing platform? Sequential has developed the gold standard test for microbiome-friendly products, in vivo (in, or on, humans). Finally, we can give some certainty about if a product is truly affecting the microbiome. We offer a complete end-to-end solution to support microbiome-friendly claims. From consultancy and study design to our proprietary microbiome testing kits. We analyse, interpret and report our findings to meet your needs. Why is it necessary to test the microbiome in vivo? At present, there are no regulations for microbiome-related formulas that brands and formulators can follow, however, it has been universally acknowledged that the in vivo method of conducting clinical studies is becoming critical and paramount to getting marketing claims through. When regulations are introduced, which may be imminent, the in vitro system will find itself lacking, resulting in limited claims and certifications that do not hold their value. This is why, we at Sequential strive to offer an in vivo approach, knowing full well that we want our client's claims to be significantly backed by scientific and quantifiable data. What type of sequencing technology does Sequential use for analysis? We offer four types of sequencing techniques including qPCR with our Smart Probes™, 16S, ITS and Shotgun Metagenomics. Using next-generation sequencing of the collection of microorganisms found on the body, during product usage, Sequential investigates the microbial diversity, and particular microorganisms we know are important and play a role in a healthy microbiome. Does Sequential offer claims certification for tested products? We provide our clients with a certification to claim “Maintains the Microbiome” subject to in vivo testing results which can be used in communication efforts. Once your product is tested with our qPCR Smart Probes™ and has shown favourable results in supporting the microbiome, we can certify your product with our Maintains the Microbiome certification seal. We have ensured that our seal and certification are backed by quantifiable data and scientifically significant markers. The aim is to ensure our clients feel confident in making their claims and can communicate the true benefit of their microbiome formulations. Unearth the secrets of your microbiome

TARGETED SKIN PANEL Acne Targeted Panel 23 Smart Probes ™ Acne Panel Validated targets: Cutibacterium acnes acnes (subtype I) Cutibacterium acnes defendens (subtype II), Cutibacterium acnes elongatum (subtype III) Absolute Quantification End-to-end Service Qet a Quote Unlock the Secrets of Acne with Sequential's Cutting-Edge Microbiome Testing Why Microbiome Testing for Acne? Acne affects nearly 85% of people between the ages of 12 and 24, with millions worldwide continuing to struggle with breakouts into adulthood (American Academy of Dermatology). While many individuals turn to over-the-counter products in hopes of finding relief, the reality is that many of these formulations can exacerbate the problem. Without proper testing, products may contain ingredients that disrupt the delicate balance of the skin, leading to irritation, clogged pores, and even more severe acne. Acne isn’t just skin-deep — it’s driven by a delicate balance of microorganisms on the skin. As skincare companies race to meet demand, a lack of microbiome-focused testing means many products fail to address the root microbial causes of acne. Sequential is leading the way in microbiome testing for the industry. With a specialized focus on skin conditions, we have developed an innovative solution tailored to help you understand the root causes of acne at the microbial level. Our proprietary sequencing panel is designed specifically to offer in-depth analysis of acne-associated microbiota, giving you the tools to transform skincare solutions. Customized Analysis Tailored insights specific to acne-prone skin, allowing your R&D team to develop more personalized and effective products. Actionable Data Leverage detailed reports to guide formulation decisions and validate product efficacy with precision. Product Innovation New acne treatments or enhancing existing lines, our data helps you create targeted solutions with proven results. Precision Sequencing Target specific microbial communities associated with acne development and severity. Smart Probe s ™ Our dedicated team of scientists has developed a groundbreaking method for evaluating microbes through our Smart Probes™, a proprietary panel featuring 23 key microbes specifically identified as having the greatest impact on skin health. To further explore the intricate connection between acne and the skin microbiome, we employ qPCR technology. This cutting-edge technique allows us to precisely measure microbial populations on the skin, providing a clearer understanding of how imbalances in the microbiome can contribute to acne breakouts. By carefully analyzing how skincare products interact with both beneficial and acne-causing microbes, companies can create solutions that not only target acne symptoms but also support a healthier, balanced skin microbiome, promoting clearer and more resilient skin. Acne Panel Targets Cutibacterium acnes acnes (Subtype I) Cutibacterium acnes subtype I is a specific strain of the C. acnes bacterium commonly found within sebaceous (oil) glands. This bacterium plays a key role in maintaining skin health by breaking down sebum, and producing fatty acids that prevent harmful pathogens from colonizing. However, certain strains of subtype I are associated with inflammatory conditions like acne vulgaris. While typically harmless, its overgrowth or imbalance in the skin microbiome can trigger immune responses that lead to acne and other skin issues. Spittaels KJ, Ongena R, Zouboulis CC, Crabbé A, Coenye T. Cutibacterium acnes Phylotype I and II Strains Interact Differently With Human Skin Cells. Front Cell Infect Microbiol. 2020 Nov 16;10:575164. doi: 10.3389/fcimb.2020.575164. PMID: 33330124; PMCID: PMC7717938. Cutibacterium acnes defendens (Subtype II) Cutibacterium acnes subtype II is a strain of the C. acnes bacterium, distinct from subtype I, and is found on human skin, primarily in sebaceous areas. While it shares similarities with other subtypes in maintaining skin health by metabolizing sebum, subtype II is less commonly associated with acne development. It has been linked more to opportunistic infections and medical device-related infections rather than inflammatory acne. Subtype II is typically less inflammatory than subtype I and plays a different role in the skin's microbial balance. Cobian N, Garlet A, Hidalgo-Cantabrana C, Barrangou R. Comparative Genomic Analyses and CRISPR-Cas Characterization of Cutibacterium acnes Provide Insights Into Genetic Diversity and Typing Applications. Front Microbiol. 2021 Nov 3;12:758749. doi: 10.3389/fmicb.2021.758749. PMID: 34803983; PMCID: PMC8595920. Cutibacterium acnes elongatum (Subtype III) Cutibacterium acnes subtype III is a less common strain of the C. acnes bacterium, primarily found in deeper skin layers and associated more with opportunistic infections rather than acne. Unlike subtypes I and II, which are typically found on the skin's surface and sebaceous areas, subtype III is linked to post-surgical infections, particularly around medical implants, and is less involved in acne formation. It tends to have lower inflammatory potential, distinguishing it from other C. acnes subtypes in terms of both location and clinical relevance. Dagnelie MA, Khammari A, Dréno B, Corvec S. Cutibacterium acnes molecular typing: time to standardize the method. Clin Microbiol Infect. 2018 Nov;24(11):1149-1155. doi: 10.1016/j.cmi.2018.03.010. Epub 2018 Mar 12. PMID: 29544912. Industry Leader in Absolute Quantification Sequential’s Smart Probe ™ qPCR approach to microbiome analysis can resolve species and subspecies, giving statistically significant findings between skin conditions on in vivo human samples, compared to conventional sequencing approaches. Relevant Research Acne & The Skin Microbiome? Unveiling Nature's Acne Assassin: The Power of Essential Oils Benzoyl Peroxide & the Skin Microbiome Read More Articles

Simplify your Study Protocols with our Tailored Candidate Recruitment Highlight your specific requirements for clinical test subjects and we will take care of the entire candidate recruitment process for you, whether it is in-person or remote. This allows us to closely supervise and guarantee the quality of microbiome collection. 10,000+ Subject Database We offer candidate recruitment across a diverse pool of candidates to give better insights into how certain formulations work with individuals of different backgrounds. In Lab or Remote Testing The nature of our recruitment process is often linked to at-home testing, however, if you would prefer a controlled environment to test participants, we can arrange for testing to be conducted within our labs in Singapore, London and New York City. Simplified Recruitment Process As a global company, we pride ourselves in our reach and ensuring your study targets are met. We can recruit all the candidates required by the study regardless of geographical barriers. Longitudinal Studies Understanding the long-term impact of your formulation of personal care products is only possible with our in vivo solution which requires human participants to test the products on themselves. Why is a Human Approach Better when it Comes to Personal Care Product Testing? At present, there are no regulations for microbiome-related formulas that brands and formulators can follow, however, it has been universally acknowledged that the in vivo method of conducting clinical studies is becoming critical to getting marketing claims through. When regulations are introduced, which may be imminent, the in vitro system will find itself lacking, resulting in limited claims and certifications that do not hold their value. This is why, we at Sequential strive to offer an in vivo approach, knowing full well that we want our client's claims to be significantly backed by scientific and quantifiable data. in vitro Lab Testing in vivo Human Testing Analyze select bacteria within a lab setting Narrow view of microbiome: Isolated from other bacteria Cannot extract meaningful conclusions on how the product will interact with its place of application Limited analysis: brief snapshot of how the product interacts with an isolated bacteria Analyze all bacteria within its natural context Broad view of microbiome: you can see interactions between bacteria Can extract meaningful conclusions on how the product will interact with its place of application Longitudinal analysis: evaluate the product over time and its impact on the microbiome Normal to Atopic Skin It’s crucial to test your product on individuals suffering through the concerns your active ingredients are targeting. This is why we believe in a fully personalized approach to candidate recruitment and human testing, bringing forward those individuals that your products are genuinely designed to help. FAQ What is Sequential's testing platform? Sequential has developed the gold standard test for microbiome-friendly products, in vivo (in, or on, humans). Finally, we can give some certainty about if a product is truly affecting the microbiome. We offer a complete end-to-end solution to support microbiome-friendly claims. From consultancy and study design to our proprietary microbiome testing kits. We analyse, interpret and report our findings to meet your needs. Why is it necessary to test the microbiome in vivo? At present, there are no regulations for microbiome-related formulas that brands and formulators can follow, however, it has been universally acknowledged that the in vivo method of conducting clinical studies is becoming critical and paramount to getting marketing claims through. When regulations are introduced, which may be imminent, the in vitro system will find itself lacking, resulting in limited claims and certifications that do not hold their value. This is why, we at Sequential strive to offer an in vivo approach, knowing full well that we want our client's claims to be significantly backed by scientific and quantifiable data. What type of sequencing technology does Sequential use for analysis? We offer four types of sequencing techniques including qPCR with our Smart Probes™, 16S, ITS and Shotgun Metagenomics. Using next-generation sequencing of the collection of microorganisms found on the body, during product usage, Sequential investigates the microbial diversity, and particular microorganisms we know are important and play a role in a healthy microbiome. Does Sequential offer claims certification for tested products? We provide our clients with a certification to claim “Maintains the Microbiome” subject to in vivo testing results which can be used in communication efforts. Once your product is tested with our qPCR Smart Probes™ and has shown favourable results in supporting the microbiome, we can certify your product with our Maintains the Microbiome certification seal. We have ensured that our seal and certification are backed by quantifiable data and scientifically significant markers. The aim is to ensure our clients feel confident in making their claims and can communicate the true benefit of their microbiome formulations.

TARGETED SKIN PANEL Malodor Targeted Panel 23 Smart Probes ™ Malodor Panel Validated targets: Corynebacterium species, Staphylococcus hominis, Cutibactrium acnes species Absolute Quantification End-to-end Service Qet a Quote Unlock the Secrets of Malodor with Sequential's Cutting-Edge Microbiome Testing Why Microbiome Testing for Malodor? Malodor, or body odor, affects millions of people globally, with studies suggesting that up to 90% of individuals experience some form of body odor during their lifetime, especially in areas such as the underarms, feet, and groin (American Society for Microbiology). This issue arises primarily due to interactions between sweat and the skin’s microbiome, where certain bacteria, like Corynebacterium and Staphylococcus , break down sweat molecules into odorous compounds. The composition of a person’s microbiome greatly influences the severity and type of body odor they experience, which highlights the need for microbiome-focused deodorant formulations. In vivo testing is crucial for deodorants because it allows scientists to see how the product affects both sweat production and the microbiome in real-world conditions. Without proper testing, deodorants might mask odors temporarily but disrupt the natural microbial balance, leading to skin irritation or increased malodour over time. By ensuring deodorants maintain a healthy microbiome, brands can create more effective, long-lasting solutions for body odor. Sequential is leading the way in microbiome testing for the industry. With a specialized focus on skin conditions, we have developed an innovative solution tailored to help you understand the root causes of malodor at the microbial level. Our proprietary sequencing panel is designed specifically to offer in-depth analysis of malodor-associated microbiota, giving you the tools to transform skincare solutions. Customized Analysis Tailored insights specific to malodor, allowing your R&D team to develop more personalized and effective products. Actionable Data Leverage detailed reports to guide formulation decisions and validate product efficacy with precision. Product Innovation New deoderants or enhancing existing lines, our data helps you create targeted solutions with proven results. Precision Sequencing Target specific microbial communities associated with malodor. Smart Probe s ™ Our dedicated team of scientists has developed a groundbreaking method for evaluating microbes through our Smart Probes™, a proprietary panel featuring 23 key microbes specifically identified as having the greatest impact on skin health, particularly in relation to malodor. To further investigate the complex relationship between body odor and the skin microbiome, we employ qPCR technology. This advanced technique enables us to precisely measure microbial populations on the skin, providing deeper insights into how imbalances in the microbiome contribute to malodor, such as the activity of bacteria like Corynebacterium and Staphylococcus . By carefully analyzing how deodorant and skincare products interact with odor-causing microbes, companies can develop solutions that not only target and reduce malodor but also support a healthy, balanced skin microbiome, ensuring long-lasting freshness and overall skin health. Malodor Panel Targets Corynebacterium species These bacteria are known to break down sweat into fatty acids and other compounds that contribute to body odor. They are particularly prevalent in areas like the armpits. Ågren MS, Ghathian KSA, Frederiksen AKS, Bjerrum MJ, Calum H, Danielsen PL, Menon J, Hædersdal M, Jorgensen LN. Zinc Oxide Inhibits Axillary Colonization by Members of the Genus Corynebacterium and Attenuates Self-perceived Malodour: A Randomized, Double-blind, Placebo-controlled Trial. Acta Derm Venereol. 2020 May 28;100(10):adv00145. doi: 10.2340/00015555-3499. PMID: 32399578; PMCID: PMC9137363. Staphylococcus hominis This bacterium is involved in the breakdown of sweat and the production of odor-causing compounds. It is commonly found in areas with high sweat gland activity. Lam, T.H., Verzotto, D., Brahma, P. et al. Understanding the microbial basis of body odor in pre-pubescent children and teenagers. Microbiome 6, 213 (2018). https://doi.org/10.1186/s40168-018-0588-z Cutibacterium acnes species Although better known for its role in acne, this bacterium also contributes to body odor by metabolizing sweat and producing odoriferous substances. Coenye T, Spittaels KJ, Achermann Y. The role of biofilm formation in the pathogenesis and antimicrobial susceptibility of Cutibacteriumacnes. Biofilm. 2021 Dec 9;4:100063. doi: 10.1016/j.bioflm.2021.100063. PMID: 34950868; PMCID: PMC8671523. Industry Leader in Absolute Quantification Sequential’s Smart Probe ™ qPCR approach to microbiome analysis can resolve species and subspecies, giving statistically significant findings between skin conditions on in vivo human samples, compared to conventional sequencing approaches. Relevant Research Unveiling the Microbial Truth: The Unpleasant Reality of Scalp Malodour Don't Sweat It: How Deodorant Disrupts Your Underarm Microbiome Read More Articles

The secrets behind your skin microbiome On the surface of your skin sits an organic ecosystem made up from billions of good bacteria called the microbiome. It forms the first layer of defense for your skin against outside forces. In order for your skin microbiome to be healthy, it must be balanced. A balanced microbiome means healthy skin What affects your skin microbiome? Your environment The country you live in can have adverse effects on your skin microbiome, from pollution levels to UV exposure. Your skin care routine When it comes to skin care, not knowing what products to use can negatively impact your skin microbiome. Your physical wellbeing What you put in your body is as important as what you put on it. A healthy diet can affect your skin health. Your mental health When you aren’t well internally, it can be a struggle to look after yourself. It is imperative to be aware of your mental health. What is Sequential's testing platform? Sequential has developed the gold standard test for microbiome-friendly products, in vivo (in, or on, humans). Finally, we can give some certainty about if a product is truly affecting the microbiome. We offer a complete end-to-end solution to support microbiome-friendly claims. From consultancy and study design to our proprietary microbiome testing kits. We analyse, interpret and report our findings to meet your needs. Why is it necessary to test the microbiome in vivo? At present, there are no regulations for microbiome-related formulas that brands and formulators can follow, however, it has been universally acknowledged that the in vivo method of conducting clinical studies is becoming critical and paramount to getting marketing claims through. When regulations are introduced, which may be imminent, the in vitro system will find itself lacking, resulting in limited claims and certifications that do not hold their value. This is why, we at Sequential strive to offer an in vivo approach, knowing full well that we want our client's claims to be significantly backed by scientific and quantifiable data. What type of sequencing technology does Sequential use for analysis? We offer four types of sequencing techniques including qPCR with our Smart Probes™, 16S, ITS and Shotgun Metagenomics. Using next-generation sequencing of the collection of microorganisms found on the body, during product usage, Sequential investigates the microbial diversity, and particular microorganisms we know are important and play a role in a healthy microbiome. Does Sequential offer claims certification for tested products? We provide our clients with a certification to claim “Maintains the Microbiome” subject to in vivo testing results which can be used in communication efforts. Once your product is tested with our qPCR Smart Probes™ and has shown favourable results in supporting the microbiome, we can certify your product with our Maintains the Microbiome certification seal. We have ensured that our seal and certification are backed by quantifiable data and scientifically significant markers. The aim is to ensure our clients feel confident in making their claims and can communicate the true benefit of their microbiome formulations. Unearth the secrets of your skin microbiome We are skin experts We are a team of award-winning scientists with a mission to empower individuals to make better-informed decisions about their skin health. Our Story

Create Your Next Best-Selling Formulation A well-designed formulation takes into account the diverse and delicate ecosystem of the microbiome. By incorporating ingredients that promote a harmonious relationship with the microbiota, such as prebiotics and postbiotics, a product can positively impact the microbial diversity and balance on the body. Having collected over 20,000+ human skin microbiome samples through in vivo clinical research, we can help you take your product from conception to completion. Consultation Book a consultation to start creating your microbiome supporting formulation. Whether you need help starting out or are already in the midst of your process, we can support you. INCI List Examination Have an INCI list and are unsure if it will be a formula? No need to look further, we will gladly look through your proposed INCI list and revert back with feedback. Co-Develop Let us be your Chief Science Officer, or your scientific advisory, and leverage our dataset for the co-development of products that suit your demographic and application. SKINCARE FORMULATIONS Regular to Atopic Skin, Start Formulating Today! What Contributes to a Microbiome Supporting Formulation? When creating a product that "maintains the microbiome", it is crucial to understand that every ingredient used is important. Hero ingredient-led philosophy cannot be applied in the formulation process as it is the blend of each ingredient that will create a product to support and improve the balance of the skin microbiome. Fermented ingredients (postbiotics) and those that act as food for microbes (prebiotics) are also known to have a positive impact on formulations when paired with the right preservative systems, surfactants, emollients, etc. It is still rare to find live bacteria (probiotics) in formulations, however, if encapsulated correctly, it could show improvement in the microbiome balance. Prebiotics Prebiotics beneficially affect the host by selectively stimulating the growth and/or activity of one or a limited number of bacteria on the skin, and thus improves host health. Prebiotics act as food for the microbes on the skin, and allow good bacteria to grow and thrive. Probiotics Probiotics are living microorganisms that confer benefit to the host when applied to the body. Because probiotics are live bacteria, it is incredibly difficult to preserve and put into skincare products (especially when skincare contains preservatives). In fact, some products on the market that say they use probiotics, are actually using prebiotics or postbiotics. Postbiotics Postbiotics are the byproduct of fermented live bacteria (probiotics) or inactivated microorganisms. The process of fermentation releases nutrients inside of the bacteria and holds concentrated and nourishing skin health benefits that help to balance the skin microbiome. INGREDIENTS FROM MICROBIAL ORIGIN No INTENDED TO BE UTILISED BY THE HUMAN MICROBIOTA? No Yes Yes ARE THEY VIABLE? No Yes ORIGIN VIABILITY FUNCTION ORDINARY INGREDIENT ORDINARY INGREDIENT WITH PREBIOTIC FUNCTION INTENDED TO BE UTILISED BY THE HUMAN MICROBIOTA? PROBIOTIC INGREDIENT No POSTBIOTIC INGREDIENT Yes POSTBIOTIC INGREDIENT WITH PREBIOTIC FUNCTION Our Leading Expert & Skincare Director Pétronille Houdart A pharmacist by training, Pétronille specialized in dermo pharmacy and cosmetology. She has garnered extensive experience in the formulation of cosmetic products and is passionate about skincare. She started her career working for a private-label contract manufacturer that focused on custom cosmetic formulations for clients ranging from big brands to private dermatologist products. She has previously created a high-performance customisable formulation brand and is vocal about bespoke and innovative cosmetic formulations. Request a Consultation Ashfi Rahman, Co-founder at HonestStory "Sequential gave us completely essential consulting work on microbiome skincare formulations. They are the only company out there that combines their knowledge of microbiome testing with formulation expertise, and we're very happy to be working with them to develop the first microbiome skincare in India." FAQ What is Sequential's testing platform? Sequential has developed the gold standard test for microbiome-friendly products, in vivo (in, or on, humans). Finally, we can give some certainty about if a product is truly affecting the microbiome. We offer a complete end-to-end solution to support microbiome-friendly claims. From consultancy and study design to our proprietary microbiome testing kits. We analyse, interpret and report our findings to meet your needs. Why is it necessary to test the microbiome in vivo? At present, there are no regulations for microbiome-related formulas that brands and formulators can follow, however, it has been universally acknowledged that the in vivo method of conducting clinical studies is becoming critical and paramount to getting marketing claims through. When regulations are introduced, which may be imminent, the in vitro system will find itself lacking, resulting in limited claims and certifications that do not hold their value. This is why, we at Sequential strive to offer an in vivo approach, knowing full well that we want our client's claims to be significantly backed by scientific and quantifiable data. What type of sequencing technology does Sequential use for analysis? We offer four types of sequencing techniques including qPCR with our Smart Probes™, 16S, ITS and Shotgun Metagenomics. Using next-generation sequencing of the collection of microorganisms found on the body, during product usage, Sequential investigates the microbial diversity, and particular microorganisms we know are important and play a role in a healthy microbiome. Does Sequential offer claims certification for tested products? We provide our clients with a certification to claim “Maintains the Microbiome” subject to in vivo testing results which can be used in communication efforts. Once your product is tested with our qPCR Smart Probes™ and has shown favourable results in supporting the microbiome, we can certify your product with our Maintains the Microbiome certification seal. We have ensured that our seal and certification are backed by quantifiable data and scientifically significant markers. The aim is to ensure our clients feel confident in making their claims and can communicate the true benefit of their microbiome formulations.