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Global Leader for in vivo Microbiome Certification Create a World of Healthier Microbiomes In today's competitive market, it's crucial to find a unique selling point that sets your brand apart from the crowd. By harnessing the power of skin microbiome science, investing in in vivo testing, and obtaining the gold standard certification, you can create a competitive advantage that resonates with your customers. Gold Standard Certification Sequential is the gold standard for in vivo microbiome testing, using the most advanced analysis and sequencing technology to extract real and impactful conclusions. Global Testing Platform Having developed a testing platform that transcends continents, Sequential’s certification is globally recognised by academics, professionals and brands across the world. Human Microbiome Testing Sequential relies solely on the in vivo (in or on humans) approach due to the complex nature of the microbiome. The only meaningful way to test the microbiome is in vivo . Proprietary Microbiome Panel Having created a simpler approach to testing with our qPCR Smart Probes™, Sequential is able to measure the impact of a product against 20 key microbes down to strain level. Why is Certification Crucial? At present, there are no legal requirements when it comes to products claiming microbiome benefits. This is because the industry is still a young one, finding its footing and establishing its norms. As a result, Sequential has developed an in vivo testing approach with 25 minimum participants per group study to ensure that our studies are acknowledged at the strictest academic level. Unlike the in vitro approach, our in vivo certification is designed to demonstrate the true impact of all formulations in a real-life context on accurate application sites. Certify your Product Today! Rising Consumer Demand for Microbiome Supporting Formulations At Sequential, we have carried out significant market research on the demand for products claiming microbiome benefits. The results of our market research on over 3,500 participants found that 80% demand a customised microbiome-based skincare product that factors ethnicity, age, location and skin concerns. Moreover, 65% understand that the skin microbiome provides a protective barrier on the surface of their skin, and 60% would spend more on a skincare product that can positively impact their skin microbiome. (Sequential market research in 2019: 99% women, ages 18+) FAQ What is Sequential's testing platform? Sequential has developed the gold standard test for microbiome-friendly products, in vivo (in, or on, humans). Finally, we can give some certainty about if a product is truly affecting the microbiome. We offer a complete end-to-end solution to support microbiome-friendly claims. From consultancy and study design to our proprietary microbiome testing kits. We analyse, interpret and report our findings to meet your needs. Why is it necessary to test the microbiome in vivo? At present, there are no regulations for microbiome-related formulas that brands and formulators can follow, however, it has been universally acknowledged that the in vivo method of conducting clinical studies is becoming critical and paramount to getting marketing claims through. When regulations are introduced, which may be imminent, the in vitro system will find itself lacking, resulting in limited claims and certifications that do not hold their value. This is why, we at Sequential strive to offer an in vivo approach, knowing full well that we want our client's claims to be significantly backed by scientific and quantifiable data. What type of sequencing technology does Sequential use for analysis? We offer four types of sequencing techniques including qPCR with our Smart Probes™, 16S, ITS and Shotgun Metagenomics. Using next-generation sequencing of the collection of microorganisms found on the body, during product usage, Sequential investigates the microbial diversity, and particular microorganisms we know are important and play a role in a healthy microbiome. Does Sequential offer claims certification for tested products? We provide our clients with a certification to claim “Maintains the Microbiome” subject to in vivo testing results which can be used in communication efforts. Once your product is tested with our qPCR Smart Probes™ and has shown favourable results in supporting the microbiome, we can certify your product with our Maintains the Microbiome certification seal. We have ensured that our seal and certification are backed by quantifiable data and scientifically significant markers. The aim is to ensure our clients feel confident in making their claims and can communicate the true benefit of their microbiome formulations.

in vivo Sampling is the Future of Microbiome Testing We lead in clinical microbiome research and testing offering a comprehensive end-to-end platform designed to bring science-backed solutions to the personal care and pharma industry. We offer an expansive platform to conduct research on personal care products through microbiome testing, biophysical assessments, and offer full recruitment services for studies. We are keen to publish our findings with our partners to increase the literature within this space. At present our database of over 20,000 human microbiome samples is one of the most sophisticated within the industry and is growing rapidly. Skin Test your skincare products, ranging from facial cleansers, serums, moisturisers, and SPFs. Scalp Test your scalp care products ranging from scalp serums, oils, shampoos, and conditioners. Vaginal Test your intimate care products, ranging from washes, to hygiene sprays, and balms. Oral Test your oral care products ranging from mouth washes and toothpastes. Full End-To-End Testing Platform in vivo Study Design Collaborating closely with our clients to develop customised study protocols, ensuring that objectives are met with precision. Validated Collection Our non-invasive collection system consistently collects material for downstream analysis and allows for remote testing. Analysis & Reporting All analysis is carried out in-house allowing for a faster turn-around time on your study with exclusive access to our team of scientists and formulation experts. White Paper Publication Co-author a white paper with us based on the findings of your study to shorten the knowledge gap within the industry to demonstrate active's impact. Candidate Recruitment Carefully select human participants (location, age, etc.) to ensure the relevance and accuracy of the study. Microbiome Detection We offer four types of sequencing techniques including qPCR with our proprietary Smart Probes™, 16S, ITS and Shotgun Metagenomics. Claims Certification Providing comprehensive reports and certifications (Maintains the Microbiome seal) based on the study results which can be used in communication efforts. Co-Presentation We offer support by co-presenting at conferences where you want to demonstrate the impact of your study findings and explain the science behind the research. Start Your Clinical Study Today! State of the Art Sequencing Using next-generation sequencing of the collection of micro-organisms found on the body, during product usage, Sequential investigates the microbial diversity, and particular micro-organisms we know are important and play a role in a healthy microbiome. With your pick from one of four sequencing reports, Sequential makes it easier to understand what depth of analysis is required for your particular needs. qPCR Microbiome (Bacteria+Fungi) Quantitative PCR (qPCR), also known as Real-Time PCR, is a method that Sequential uses in-house to measure the absolute number of copies of a microbial DNA defined by Sequential’s proprietary Smart Probes™. Using this method, we quantify the amount bacteria or fungi, allowing us to report level of species and strains within a given sample. The entire process is done at Sequential to ensure quality control, and extra control on your samples and privacy. Up Next-Generation Sequencing of ITS (total fungal population) ITS rRNA gene sequencing, or ITS amplicon sequencing is performed at Sequential to determine the relative abundance of taxa in a fungal community, and to compare between groups of interest. We know that fungi play a critical role in the microbiome of a healthy human, and it’s important to consider this application, especially for the scalp microbiome, and vaginal or vulva microbiome. We routinely offer two types of ITS rRNA primer regions: ITS1 & ITS2 Up Next-Generation Sequencing of 16S 16S rRNA gene sequencing, or 16S amplicon sequencing, is performed at Sequential to determine the relative abundance of taxa in a bacterial community, and to compare between groups of interest. This level of analysis can help to address changes in the overall bacterial profile over time or between treatment groups. We routinely offer two types of 16s rRNA primer regions: 16S V4 & 16S V1-V3 Up Shotgun Metagenomic Sequencing Shotgun metagenome sequencing is performed at Sequential for taxonomic profiling (diversity and abundance), as well as functional analysis across different organisms in a more exploratory approach. This complex technique allows for parallel sequencing of DNA from all organisms (bacterial, fungal, viral and human) within the community, with good coverage for species-level detection. The data generation allows for more advanced reporting and genome assemblies. Up FAQ What is Sequential's testing platform? Sequential has developed the gold standard test for microbiome-friendly products, in vivo (in, or on, humans). Finally, we can give some certainty about if a product is truly affecting the microbiome. We offer a complete end-to-end solution to support microbiome-friendly claims. From consultancy and study design to our proprietary microbiome testing kits. We analyse, interpret and report our findings to meet your needs. Why is it necessary to test the microbiome in vivo? At present, there are no regulations for microbiome-related formulas that brands and formulators can follow, however, it has been universally acknowledged that the in vivo method of conducting clinical studies is becoming critical and paramount to getting marketing claims through. When regulations are introduced, which may be imminent, the in vitro system will find itself lacking, resulting in limited claims and certifications that do not hold their value. This is why, we at Sequential strive to offer an in vivo approach, knowing full well that we want our client's claims to be significantly backed by scientific and quantifiable data. What type of sequencing technology does Sequential use for analysis? We offer four types of sequencing techniques including qPCR with our Smart Probes™, 16S, ITS and Shotgun Metagenomics. Using next-generation sequencing of the collection of microorganisms found on the body, during product usage, Sequential investigates the microbial diversity, and particular microorganisms we know are important and play a role in a healthy microbiome. Does Sequential offer claims certification for tested products? We provide our clients with a certification to claim “Maintains the Microbiome” subject to in vivo testing results which can be used in communication efforts. Once your product is tested with our qPCR Smart Probes™ and has shown favourable results in supporting the microbiome, we can certify your product with our Maintains the Microbiome certification seal. We have ensured that our seal and certification are backed by quantifiable data and scientifically significant markers. The aim is to ensure our clients feel confident in making their claims and can communicate the true benefit of their microbiome formulations.

Cookie Policy Last updated: 13 March 2024 Sequential Skin Ltd ("us", "we", or "our") operates the www.sequential.bio website (the "Service"). This page informs you of our policies regarding the collection, use, and disclosure of personal data when you use our Service and the choices you have associated with that data. We use your data to provide and improve the Service. By using the Service, you agree to the collection and use of information in accordance with this policy. Unless otherwise defined in this Cookie Policy, the terms used in this Cookie Policy have the same meanings as in our Privacy Policy. Definitions Service: Service is the www.sequential.bio website operated by Sequential. Personal Data: Personal Data means data about a living individual who can be identified from those data (or from those and other information either in our possession or likely to come into our possession). Usage Data: Usage Data is data collected automatically either generated by the use of the Service or from the Service infrastructure itself (for example, the duration of a page visit). Cookies: Cookies are small pieces of data stored on your device (computer or mobile device). Data Controller: Data Controller means the natural or legal person who (either alone or jointly or in common with other persons) determines the purposes for which and the manner in which any personal information are, or are to be, processed. For the purpose of this Privacy Policy, we are a Data Controller of your Personal Data. Data Processors (or Service Providers): Data Processor (or Service Provider) means any natural or legal person who processes the data on behalf of the Data Controller. We may use the services of various Service Providers in order to process your data more effectively. Data Subject (or User): Data Subject is any living individual who is using our Service and is the subject of Personal Data. Information Collection and Use We use cookies and similar tracking technologies to track the activity on our Service and hold certain information. Types of Cookies We Use Session Cookies: We use Session Cookies to operate our Service. Preference Cookies: We use Preference Cookies to remember your preferences and various settings. Security Cookies: We use Security Cookies for security purposes. Advertising Cookies: Advertising Cookies are used to serve you with advertisements that may be relevant to you and your interests. Use of Data We use the collected data for various purposes: To provide and maintain our Service To notify you about changes to our Service To allow you to participate in interactive features of our Service when you choose to do so To provide customer support To gather analysis or valuable information so that we can improve our Service To monitor the usage of our Service To detect, prevent and address technical issues Retention of Data We will retain your Personal Data only for as long as is necessary for the purposes set out in this Cookie Policy. We will retain and use your Personal Data to the extent necessary to comply with our legal obligations (for example, if we are required to retain your data to comply with applicable laws), resolve disputes, and enforce our legal agreements and policies. Your Choices Regarding Cookies If you'd like to delete cookies or instruct your web browser to delete or refuse cookies, please visit the help pages of your web browser. Please note, however, that if you delete cookies or refuse to accept them, you might not be able to use all of the features we offer, you may not be able to store your preferences, and some of our pages might not display properly. Service Providers We may employ third party companies and individuals to facilitate our Service ("Service Providers"), provide the Service on our behalf, perform Service-related services, or assist us in analyzing how our Service is used. These third parties have access to your Personal Data only to perform these tasks on our behalf and are obligated not to disclose or use it for any other purpose. Changes to This Cookie Policy We may update our Cookie Policy from time to time. We will notify you of any changes by posting the new Cookie Policy on this page. You are advised to review this Cookie Policy periodically for any changes. Changes to this Cookie Policy are effective when they are posted on this page. Contact Us If you have any questions about this Cookie Policy, please contact us: By email: team@sequential.bio By visiting this page on our website: www.sequential.bio

Unveiling Sequential Bio’s New London Lab testing HQ 18th October 2022 - In an effort to keeping the entire testing process in-house, allowing for rigorous quality control, and meeting the demand of our European clients - today we announce the opening of our testing lab in London. Sequential is proud to announce that it will open a European HQ, and testing lab site in White City, London. One key quality that makes Sequential stand out from its competitors is keeping the testing protocols and quantitative analysis in-house. This gives them the ability to ensure the processing, and targeting of microbiome markers for their clients is extremely high quality. “Innovative R&D, and rigorous quality control on our lab testing and processes have always been front of mind at Sequential. By opening a lab in London, we’re responding to the demand from customers - whilst retaining control over our quality in testing.” - Dr Oliver Worsley, CEO/Co-Founder. Adding to their testing labs in Singapore, and New York, in which they serve Singapore, Thailand, Australia, and the US markets. This enables faster turnaround time for European clients, as the demand for non-invasive molecular analysis of the skin reaches new heights in Europe. Between 2018 - 2022, there have been over 34 microbiome deals (licensing and investments) made. With an average contract value of $257M, this has created over $2.3B of value in the gut and skin microbiome space. Europe is a significant player in the global microbiome field, expected to grow at a CAGR of 24.25% (between 2022-2028). About Sequential Sequential is part of the Sequential Skin group – with a US lab in New York City, alongside a lab in London and a lab in Singapore. Sequential Bio team has over 20 years of combined expertise in genetics, epigenetics, and microbiome research. It was recognised recently as the “Most Significant ” Testing Method in the Industry. Sequential has validated its AI-driven testing platform with over 30 companies, analyzing over 12,000 skin microbiome samples. They specialise in skin, scalp and vulva/vaginal microbiome samples. They are supported by Enterprise SG, A*STAR, Genome Institute of Singapore, IndieBio New York, SOSV, Metaplanet Holdings, Scrum Ventures, Genedant VC, Ben Holmes (ex. General Partner at Index Ventures), Innovate UK, and are a resident company of Johnson & Johnson Innovation – JLABS.

TARGETED SKIN PANEL Sun/UV Targeted Panel 23 Smart Probes ™ Sun/UV Panel Validated targets: Sphingomonas spp, Cyanobacteria spp, Malassezia furfur Absolute Quantification End-to-end Service Qet a Quote Unlock the Secrets of UV Exposure with Sequential's Cutting-Edge Microbiome Testing Why Microbiome Testing for SPF Products? According to the Skin Cancer Foundation, about 1 in 5 Americans will develop skin cancer by the age of 70, with UV exposure being one of the most common causes. With the growing awareness of the risks associated with UV damage, in vivo testing for SPF products has become increasingly crucial. The skin microbiome plays a pivotal role in protecting against UV radiation, but excessive sun exposure can disrupt this delicate balance, leading to increased risk for skin cancer and other forms of skin damage. By conducting in vivo testing, companies can better assess how sunscreen and SPF products interact with the microbiome, ensuring not only UV protection but also the maintenance of a healthy skin barrier. This holistic approach is essential to create safer, more effective sun-care products that prevent long-term damage (American Academy of Dermatology). Sequential is leading the way in microbiome testing for the industry. With a specialized focus on skin conditions, we have developed an innovative solution tailored to help you understand the root causes of UV exposure at the microbial level. Our proprietary sequencing panel is designed specifically to offer in-depth analysis of sun-exposed microbiota, giving you the tools to transform skincare solutions. Customized Analysis Tailored insights specific to UV exposure, allowing your R&D team to develop more personalized and effective products. Actionable Data Leverage detailed reports to guide formulation decisions and validate product efficacy with precision. Product Innovation New SPF products or enhancing existing lines, our data helps you create targeted solutions with proven results. Precision Sequencing Target specific microbial communities associated with sun exposure and UV related skin conditions. Smart Probe s ™ Our dedicated team of scientists has developed an innovative method for evaluating the effects of sun exposure and UV radiation on the skin using our Smart Probes™ technology. This proprietary panel features 23 key markers that we’ve identified as having the most significant influence on skin health, particularly in response to UV damage. To further understand the impact of UV radiation on the skin’s microbiome and overall health, we utilize qPCR technology. This advanced technique allows us to accurately measure changes in microbial populations and skin health following sun exposure. By closely analyzing how sun care products interact with these key markers and microbes, companies can develop solutions that not only protect against UV damage but also support the skin’s natural defenses, ensuring long-term skin health and resilience against sun-induced aging and other effects. Sun/UV Panel Targets Sphingomonas Sphingomonas has been shown to be highly resistant to UV irradiation and capable of reducing reactive oxygen species (ROS) levels in human keratinocytes—the predominant cell type in the epidermis. This discovery underscores the important role that members of the skin microbiome, such as Sphingomonas , play in protecting human skin from solar radiation damage. By mitigating oxidative stress caused by UV exposure, Sphingomonas may contribute to healthier skin and potentially enhance the skin's natural defense mechanisms. Harel N, Ogen-Shtern N, Reshef L, Biran D, Ron EZ, Gophna U. Skin microbiome bacteria enriched following long sun exposure can reduce oxidative damage. Res Microbiol. 2023 Nov-Dec;174(8):104138. doi: 10.1016/j.resmic.2023.104138. Epub 2023 Sep 16. PMID: 37722498. Cyanobacteria Cyanobacteria have shown potential in skin care due to their ability to decrease pigmentation and reduce damage associated with photoaging. According to research, Cyanobacteria on the skin surface can help mitigate these effects by lowering melanin production and protect the skin from oxidative stress, which contributes to the aging process. Skowron K, Bauza-Kaszewska J, Kraszewska Z, Wiktorczyk-Kapischke N, Grudlewska-Buda K, Kwiecińska-Piróg J, Wałecka-Zacharska E, Radtke L, Gospodarek-Komkowska E. Human Skin Microbiome: Impact of Intrinsic and Extrinsic Factors on Skin Microbiota. Microorganisms. 2021 Mar 5;9(3):543. doi: 10.3390/microorganisms9030543. PMID: 33808031; PMCID: PMC7998121. Malassezia furfur Malassezia furfur is a species of yeast commonly found on human skin, often associated with various dermatological conditions. Despite its ability to synthesize a UV-protective substance called pityriacitrin, Malassezia furfur demonstrates high sensitivity to ultraviolet (UV) radiation. Burns EM, Ahmed H, Isedeh PN, Kohli I, Van Der Pol W, Shaheen A, Muzaffar AF, Al-Sadek C, Foy TM, Abdelgawwad MS, Huda S, Lim HW, Hamzavi I, Bae S, Morrow CD, Elmets CA, Yusuf N. Ultraviolet radiation, both UVA and UVB, influences the composition of the skin microbiome. Exp Dermatol. 2019 Feb;28(2):136-141. doi: 10.1111/exd.13854. Epub 2019 Jan 14. PMID: 30506967; PMCID: PMC7394481. Industry Leader in Absolute Quantification Sequential’s Smart Probe ™ qPCR approach to microbiome analysis can resolve species and subspecies, giving statistically significant findings between skin conditions on in vivo human samples, compared to conventional sequencing approaches. Relevant Research Shedding Light on Summer Sun: Does UV Exposure Affect the Skin Microbiome? Read More Articles

Vaginal & Vulvar Microbiome Testing As the intimate care industry grows, consumers demand transparency from the brands formulating their products. It has become essential for formulators to create products they are willing to test to present scientifically backed data-driven evidence of their products' true effects. Sequential offers microbiome testing for your intimate care formulations, hygiene washes and other products geared towards that delicate area. We are dedicated to understanding how your product interacts with the vaginal region and its microbes. Depending on how in-depth you want to go, we offer qPCR, 16S, ITS, and Shotgun Metagenomics. Download Case Study! Personalized Approach to Testing Unlike other methodologies present within the industry, Sequential's approach ensures that your product's data and analysis will stand the test of industry regulations when they are introduced. You can tailor your study entirely to your unique requirements. Test Products in a Real-Life Context The microbiome comprises a complex ecosystem of microorganisms that live together in a delicate balance, which is why it's best to test directly upon it directly. To fully understand the impact a product is having on the microbiome, in vivo is the only way. Collect Longitudinal Data With in vivo testing we can design your study around the extended use of a product over multiple time points. This allows us to review how a product is performing before and after usage, but also take into account its gradual impact on the microbiome. Measure Against a Control Group Measure a product against a control group that might have a different percentage of your active ingredient within its formulation or no active at all. This will allow for deeper insights into the impact of a formulation on microbial balance and diversity. 4 Sequencing Reports To Pick From Depending on your development stage and what you are interested in studying we offer qPCR, 16S, ITS, and Shotgun Metagenomics. With our qPCR Smart Probes™ we can go down to the strain level in our analysis. Personalize Your Microbiome Study! The vaginal microbiome is a dynamic ecosystem primarily dominated by Lactobacillus bacteria, which produce lactic acid and help maintain an acidic pH, creating a hostile environment for potential pathogens. This acidic environment is crucial for preventing infections and promoting overall vaginal health. Smart Probe s ™ Our dedicated team of scientists have developed a method of evaluating microbes through our Smart Probes ™ . These refer to a panel of 20 key microbes we have specifically identified as having the most impact on vaginal health. Over and above the taxonomic characterisation that 16S offers, which gives us a snapshot of all the genus present within a collected sample (Lactobacillus , Streptococcus , etc.) our targeted approach takes it a step further, opening the lens to the species (L.crispatus ), sub-species and even strains within them. This is a crucial distinction as not all strains of a species behave similarly. In fact, we find that within these species there are strains associated with inflammation and strains that are commensal, and beneficial. Gold Standard Certification Sequential has developed the gold standard test for products designed to target the microbiome, in vivo (in, or on, humans). Finally, we can give some certainty about if a product is truly affecting the microbiome. Using next-generation sequencing of the collection of micro-organisms found on the body, before and after product usage, Sequential investigates the microbial balance and diversity, and particular micro-organisms we know are important and play a role in a healthy microbiome. We give you an in vivo certification that your product maintains the microbiome. And it’s not exclusive to skincare! We do this for haircare products, oral products, and vulva/vaginal microbiomes. Personalize Your Microbiome Study! Supplement Your Microbiome Study Recruitment Services Let us take care of the entire candidate recruitment process for you! View More Biophysical Assessments Increase your data on the use of your product by evaluating additional biophysical factors. View More Formulation Support Seek consultation advice for your formulation if you are re-formulating or developing a new product. View More FAQ What is Sequential's testing platform? Sequential has developed the gold standard test for microbiome-friendly products, in vivo (in, or on, humans). Finally, we can give some certainty about if a product is truly affecting the microbiome. We offer a complete end-to-end solution to support microbiome-friendly claims. From consultancy and study design to our proprietary microbiome testing kits. We analyse, interpret and report our findings to meet your needs. Why is it necessary to test the microbiome in vivo? At present, there are no regulations for microbiome-related formulas that brands and formulators can follow, however, it has been universally acknowledged that the in vivo method of conducting clinical studies is becoming critical and paramount to getting marketing claims through. When regulations are introduced, which may be imminent, the in vitro system will find itself lacking, resulting in limited claims and certifications that do not hold their value. This is why, we at Sequential strive to offer an in vivo approach, knowing full well that we want our client's claims to be significantly backed by scientific and quantifiable data. What type of sequencing technology does Sequential use for analysis? We offer four types of sequencing techniques including qPCR with our Smart Probes™, 16S, ITS and Shotgun Metagenomics. Using next-generation sequencing of the collection of microorganisms found on the body, during product usage, Sequential investigates the microbial diversity, and particular microorganisms we know are important and play a role in a healthy microbiome. Does Sequential offer claims certification for tested products? We provide our clients with a certification to claim “Maintains the Microbiome” subject to in vivo testing results which can be used in communication efforts. Once your product is tested with our qPCR Smart Probes™ and has shown favourable results in supporting the microbiome, we can certify your product with our Maintains the Microbiome certification seal. We have ensured that our seal and certification are backed by quantifiable data and scientifically significant markers. The aim is to ensure our clients feel confident in making their claims and can communicate the true benefit of their microbiome formulations.

Privacy Policy 1. Introduction 1.1 Important information and who we are Welcome to Sequential Skin Ltd’s Privacy and Data Protection Policy (“Privacy Policy”). At Sequential Skin Ltd (“we”, “us”, or “our”) we are committed to protecting and respecting your privacy and Personal Data in compliance with the United Kingdom General Data Protection Regulation (“GDPR”), the Data Protection Act 2018 and all other mandatory laws and regulations of the United Kingdom. This Privacy Policy explains how we collect, process and keep your data safe. The Privacy Policy will tell you about your privacy rights, how the law protects you, and inform our employees and staff members of all their obligations and protocols when processing data. The individuals from which we may gather and use data can include: Customers Suppliers Business contacts Employees/Staff Members Third parties connected to your customers Collaborators, academic institutes, industry partners. and any other people that the organisation has a relationship with or may need to contact. This Privacy Policy applies to all our employees and staff members and all Personal Data processed at any time by us. 1.2 Your Data Controller Sequential Skin Ltd is your Data Controller and responsible for your Personal Data. We are not obliged by the GDPR to appoint a data protection officer and have not voluntarily appointed one at this time. Therefore, any inquiries about your data should either be sent to us by email to team@sequential.bio or by post to 85 Great Portland Street, London, W1W 7LT, United Kingdom. You have the right to make a complaint at any time to the Information Commissioner’s Office (ICO), the UK supervisory authority for data protection issues (www.ico.org.uk ). We would, however, appreciate the chance to deal with your concerns before you approach the ICO so please contact us in the first instance. 1.3 Processing data on behalf of a Controller and processors’ responsibility to you In discharging our responsibilities as a Data Controller we have employees who will deal with your data on our behalf (known as “Processors”). The responsibilities below may be assigned to an individual or may be taken to apply to the organisation as a whole. The Data Controller and our Processors have the following responsibilities: Ensure that all processing of Personal Data is governed by one of the legal bases laid out in the GDPR (see 2.2 below for more information); Ensure that Processors authorised to process Personal Data have committed themselves to confidentiality or are under an appropriate statutory obligation of confidentiality; Implement appropriate technical and organisational measures to ensure a level of security appropriate to the risk associated with the processing of Personal Data; Obtain the prior specific or general authorisation of the Controller before engaging another Processor; Assist the Controller in the fulfilment of the Controller's obligation to respond to requests for exercising the data subject's rights; Make available to the Controller all information necessary to demonstrate compliance with the obligations laid down in the GDPR and allow for and contribute to audits, including inspections, conducted by the Controller or another auditor mandated by the Controller; Maintain a record of all categories of processing activities carried out on behalf of a Controller; Cooperate, on request, with the supervisory authority in the performance of its tasks; Ensure that any person acting under the authority of the Processor who has access to Personal Data does not process Personal Data except on instructions from the Controller; and Notify the Controller without undue delay after becoming aware of a Personal Data Breach. 2. Legal Basis for Data Collection 2.1 Types of data / Privacy policy scope “Personal Data” means any information about an individual from which that person can be identified. It does not include data where the identity has been removed (anonymous data). We may collect, use, store and transfer different kinds of Personal Data about you which we have grouped together below. Not all of the following types of data will necessarily be collected from you but this is the full scope of data that we collect and when we collect it from you: Profile/Identity Data: This is data relating to your first name, last name, gender, date of birth. Contact Data: This is data relating to your phone number, addresses, email addresses, phone numbers. Marketing and Communications Data: This is your preferences in receiving marketing information and other information from us. Technical Data: This is your IP address, browser type and version, time zone setting and location, operating system and platform, and other technology on the devices you use to engage with us. Customer Support Data: This includes feedback and survey responses. Usage Data: information about how you use our website, products and services. We do not collect any Special Categories of Personal Data about you (this includes details about your race or ethnicity, religious or philosophical beliefs, sex life, sexual orientation, political opinions, trade union membership, information about your health, and genetic and biometric data). Nor do we collect any information about criminal convictions and offences. 2.2 The Legal Basis for Collecting That Data There are a number of justifiable reasons under the GDPR that allow collection and processing of Personal Data. The main avenues we rely on are: “Consent”: Certain situations allow us to collect your Personal Data, such as when you tick a box that confirms you are happy to receive email newsletters from us, or ‘opt in’ to a service. “Contractual Obligations”: We may require certain information from you in order to fulfil our contractual obligations and provide you with the promised service. “Legal Compliance”: We’re required by law to collect and process certain types of data, such as fraudulent activity or other illegal actions. “Legitimate Interest”: We might need to collect certain information from you to be able to meet our legitimate interests - this covers aspects that can be reasonably expected as part of running our business, that will not have a material impact on your rights, freedom or interests. Examples could be your address, so that we know where to deliver something to, or your name, so that we have a record of who to contact moving forwards. 3. How We Use your Personal Data 3.1 Our data uses We will only use your Personal Data when the law allows us to. Set out below is a table containing the different types of Personal Data we collect and the lawful basis for processing that data. Please refer to section 2.2 for more information on the lawful basis listed in the table below. Examples provided in the table below are indicative in nature and the purposes for which we use your data may be broader than described but we will never process your data without a legal basis for doing so and it is for a related purpose. For further inquiries please contact us. 3.2 Marketing and content updates You will receive marketing and new content communications from us if you have created an account and chosen to opt into receiving those communications. From time to time we may make suggestions and recommendations to you about goods or services that may be of interest to you. 3.3 Change of purpose We will only use your Personal Data for the purposes for which we collected it, unless we reasonably consider that we need to use it for another reason and that reason is compatible with the original purpose. If you wish to get an explanation as to how the processing for the new purpose is compatible with the original purpose, please contact us. If we need to use your Personal Data for an unrelated purpose, we will notify you and we will explain the legal basis which allows us to do so. Please note that we may process your Personal Data without your knowledge or consent, in compliance with the above rules, where this is required or permitted by law. 4. Your Rights and How You Are Protected By Us 4.1 Your Legal Rights Under certain circumstances, you have the following rights under data protection laws in relation to your personal data: Right to be informed. You have a right to be informed about our purposes for processing your personal data, how long we store it for, and who it will be shared with. We have provided this information to you in this policy. Right of access. This enables you to receive a copy of the personal data we hold about you and to check that we are lawfully processing it (also known as a "data subject access request"). See section 4.4 below for more details on how you can make a data subject access request. Right to rectification. You have a right to request correction of the personal data that we hold about you. This enables you to have any incomplete or inaccurate data we hold about you corrected, though we may need to verify the accuracy of the new data you provide to us. Right to erasure. You have the right to ask us to delete or remove personal data where there is no good reason for us continuing to process it, where you have successfully exercised your right to object to processing (see below), where we may have processed your information unlawfully or where we are required to erase your personal data to comply with local law. Note, however, that we may not always be able to comply with your request of erasure for specific legal reasons which will be notified to you, if applicable, at the time of your request. Right to object. You can object to the processing of personal data we hold about you. This effectively allows you to stop or prevent us from processing your personal data. Note that this is not an absolute right and it only applies in certain circumstances, for example: (i) Where we are processing your personal data for direct marketing purposes. (ii) Where we are relying on a legitimate interest (or those of a third party) and there is something about your particular situation which makes you want to object to processing on this ground as you feel it impacts on your fundamental rights and freedoms. (iii) In some cases, we may continue processing your data if we can demonstrate that we have compelling legitimate grounds to process your information which override your rights and freedoms. Right to restrict processing. You have the right to request the restriction or suppression of their personal data. Note that this is not an absolute right and it only applies in certain circumstances: (i) If you want us to establish the data's accuracy. (ii) Where our use of the data is unlawful but you do not want us to erase it. (iii) Where you need us to hold the data even if we no longer require it as you need it to establish, exercise or defend legal claims. (iv) You have objected to our use of your data but we need to verify whether we have overriding legitimate grounds to use it. Right to data portability. You have the right to request the transfer of your personal data to you or to a third party. If you make such a request, we will provide to you, or a third party you have chosen, your personal data in a structured, commonly used, machine-readable format. Note that this right only applies to automated information which you initially provided consent for us to use or where we used the information to perform a contract with you. If you wish to make a request under any of these rights, please contact us at team@sequential.bio . 4.2 How Sequential Skin Ltd protects customers' Personal Data We are concerned with keeping your data secure and protecting it from inappropriate disclosure. We implement a variety of security measures to ensure the security of your Personal Data on our systems, including We implement certain security measures to ensure the security of your Personal Data on our systems, including using encrypted servers that gives confidence that personal data is being treated securely.. Any Personal Data collected by us is only accessible by a limited number of employees who have special access rights to such systems and are bound by obligations of confidentiality. If and when we use subcontractors to store your data, we will not relinquish control of your Personal Data or expose it to security risks that would not have arisen had the data remained in our possession. However, unfortunately no transmission of data over the internet is guaranteed to be completely secure. It may be possible for third parties not under the control of Sequential Skin Ltd to intercept or access transmissions or private communications unlawfully. While we strive to protect your Personal Data, we cannot ensure or warrant the security of any Personal Data you transmit to us. Any such transmission is done at your own risk. If you believe that your interaction with us is no longer secure, please contact us. 4.3 Opting out of marketing promotions You can ask us to stop sending you marketing messages at any time by unsubscribing at the bottom of the email. Where you opt out of receiving these marketing messages, we will continue to retain other Personal Data provided to us as a result of interactions with us not related to your marketing preferences. 4.4 How to request your data and the process for obtaining it You will not have to pay a fee to access your Personal Data (or to exercise any of the other rights). However, if your request is clearly unfounded, we could refuse to comply with your request. We may need to request specific information from you to help us confirm your identity and ensure you have the right to access your Personal Data (or to exercise any of your other rights). This is a security measure to ensure that Personal Data is not disclosed to any person who has no right to receive it. We may also contact you to ask you for further information in relation to your request to speed up our response. 5. Your Data and Third Parties 5.1 Sharing your data with third parties We may share non-Personal Data with third parties. We may share your Personal Data with subcontractors or affiliates, subject to confidentiality obligations to use it only for the purposes for which we disclose it to them and pursuant to our instructions. We may also share Personal Data with interested parties in the event that Sequential Skin Ltd anticipates a change in control or the acquisition of all or part of our business or assets or with interested parties in connection with the licensing of our technology. If Sequential Skin Ltd is sold or makes a sale or transfer, we may, in our sole discretion, transfer, sell or assign your Personal Data to a third party as part of or in connection with that transaction. Upon such transfer, the Privacy Policy of the acquiring entity may govern the further use of your Personal Data. In all other situations your data will still remain protected in accordance with this Privacy Policy (as amended from time to time). We may share your Personal Data at any time if required for legal reasons or in order to enforce our terms or this Privacy Policy. 5.2 Third-Party Links This Site may include links to third-party websites, plug-ins and applications. Clicking on those links or enabling those connections may allow third parties to collect or share data about you. We do not control these third-party websites and are not responsible for their privacy statements. When you leave our Site, we encourage you to read the privacy policy of every website you visit. 6. How Long We Retain Your information We will only retain your Personal Data for as long as reasonably necessary to fulfil the purposes we collected it for. We may retain your Personal Data for a longer period than usual in the event of a complaint or if we reasonably believe there is a prospect of litigation in respect to our relationship with you. 7. International Transfer of Data Your information may be stored and processed in the US or other countries or jurisdictions outside the US where Sequential Skin Ltd has facilities. By using Sequential Skin Ltd, you are permitting and consenting to the transfer of information, including Personal Data, outside of the US. 8. Notification of Changes and Acceptance of Policy We keep our Privacy Policy under review and will place any updates here. This version is dated 23 February 2024. By using Sequential Skin Ltd, you consent to the collection and use of data by us as set out in this Privacy Policy. Continued access or use of Sequential Skin Ltd will constitute your express acceptance of any modifications to this Privacy Policy. 9. Interpretation All uses of the word "including" mean "including but not limited to" and the enumerated examples are not intended to in any way limit the term which they serve to illustrate. Any email addresses set out in this policy may be used solely for the purpose for which they are stated to be provided, and any unrelated correspondence will be ignored. Unless otherwise required by law, we reserve the right to not respond to emails, even if they relate to a legitimate subject matter for which we have provided an email address. You are more likely to get a reply if your request or question is polite, reasonable and there is no relatively obvious other way to deal with or answer your concern or question (e.g. FAQs, other areas of our website, etc.). Our staff are not authorised to contract on behalf of Sequential Skin Ltd, waive rights or make representations (whether contractual or otherwise). If anything contained in an email from a Sequential Skin Ltd address contradicts anything in this policy, our terms or any official public announcement on our website, or is inconsistent with or amounts to a waiver of any Sequential Skin Ltd rights, the email content will be read down to grant precedence to the latter. The only exception to this is genuine correspondence expressed to be from the Sequential Skin Ltd legal department.

Allē Award: Sequential Wins Prestigious “Most Significant” Testing Method After being listed as a finalist as best ‘Claims testing methods & tools’ alongside Evonik, and XCellR8 - Sequential brings home top prize in C&T’s Allē Awards, 2022. The personal care and cosmetics industry in the US is valued at $190B. In the context of the skin microbiome, the industry is still a relatively nascent field. However, its projected growth is significant, estimated to grow at a compounded annual growth of 29.2% between 2022-2028. Owed to the impact of products on the skin microbiome, consumer awareness, and understanding that our microbiome is intimately linked to our health. Sequential is the B2B microbiome testing arm for Sequential Skin Ltd, which has developed the world’s first end-to-end platform for companies to evaluate their products on the microbiome in vivo. They specialize in skin, scalp and intimate area microbiome. "Of all the 'microbiome friendly' methodologies working to address both consumer and industry concerns about the impact of cosmetics on the microbiome, this is, in my opinion, the most quantitative and promising of the lot." – C&T's Allē Award judges, 2022. The company has developed a proprietary non-invasive method for collecting skin samples to analyze the skin microbiome using next-generation sequencing (NGS), with species and strain identification, in longitudinal (and clinical) studies. Services include data interpretation and the results are reported in a comprehensive, yet understandable format, ready for formulators and research scientists to incorporate into their product development pipeline. “The Allē Award gives us strong confidence in the testing platform and capabilities we’ve built in Sequential. Being the first to develop an in vivo microbiome test for the industry, we’re proud to see our hard work paying off” — Petronille Houdart, DPharm, skincare director at Sequential. About Sequential Sequential is part of the Sequential Skin group – with a US lab in New York City, alongside a lab in London and a lab in Singapore. Sequential team has over 20 years of combined expertise in genetics, epigenetics, and microbiome research. Sequential has validated its AI-driven testing platform with over 30 companies, analyzing over 12,000 skin microbiome samples. They specialise in skin, scalp and vulva/vaginal microbiome samples. They are supported by Enterprise SG, A*STAR, Genome Institute of Singapore, IndieBio New York, SOSV, Metaplanet Holdings, Scrum Ventures, Genedant VC, Ben Holmes (ex. General Partner at Index Ventures), Innovate UK, and are a resident company of Johnson & Johnson Innovation – JLABS.

Category Ingredient What is it? Benefit Melanin Targeters Arbutin Arbutin is an antioxidant that is extracted from different berries. Arbutin helps in ensuring that skin is brightened and pigmentation is reduced in the skin. It can also fade scars. Hydrators Squalane Squalane is a hydrogenated version of squalene. It is an emollient and behaves similar to our own skin sebum. Prevents moisture loss, can neutralize UV damage . Postbiotics Lactobacillus ferment Lactobacillus ferment is a non-living probiotic ingredient made by fermentation of the microorganism Lactobacillus. Thanks to its soothing effect on the skin, it helps to strengthen the skin's defense and maintains the health of the skin microbiome. Allantoin Allantoin is an extract from comfrey plant. Allantoin protects and soothes the skin. Trehalose Trehalose is a disaccharide with hydrating properties for the skin. It’s a plant sugar. Trehalose is used as a prebiotic and is effective to help maintain healthy skin. Green Tea Extract Extract from the leaves of green tea. Tea Extracts is an antioxidant ingredient that aids soothing the skin and can also address skin discoloration and early signs of aging. Colloidal Oatmeal Colloidal Oatmeal is cocktail of oats that has been refined into powder and acts as a skin protectant. Colloidal Oatmeal aids in locking in moisture in the skin barrier and also has soothing properties. Skin Soothing Multi-Ceramide [Ceramide NP; Phytosphingosine] Ceramides are lipids or fats that exist in skin cells and make up to 40% of your outer skin layer. Ceramides are crucial in aiding your skin to retain moisture and prevent unwanted bacteria from entering your body. Skin Repairing Peptide Complex [copper tripeptide-1, Acetyl heaxapeptide-8, Palmitoyl Pentapepdide-4, Palmitoyl tripeptide-1, Palmitoyl tetrapepide-7, tripeptide-1, tripeptide-2, tripeptide-2, tripeptide-3, acetyl Octapeptide 3] Peptides are amino acid chains that aid in the forming of certain types of protein in the skin such as elastin and collagen. Peptides can help in increasing skin elasticity and reducing signs of aging, allowing skin to appear firmer. Skin Shields Vitamin E [Tocopherol] Tocopherol is an excellent example of a powerful antioxidant that is oil-soluble, meaning it can easily dissolve into the skin. Tocopherol helps in combating free-radical damage (such as pollution), and can also be an effective hydrator. Retinyl Palmitate Retinyl Palmitate is the most abundantly used form of retinol (Vitamin A). Retinyl Palmitate has anti-aging properties as well as anti-oxidant properties. Centella Asiatica Centella Asiatica is a medicinal herb that is known to treat inflammation and has antioxidant properties. Centella Asiatica is used as an active ingredient in skincare products for anti-inflammation, anti-oxidant and soothes compromised skin. Sorbitol Sorbitol is a sugar with hydrating properties that can nourish the probiotics that live on the skin allowing the skin microbiome to be balanced. Sorbitol breakdown by microorganisms on the skin creates beta-glucan, a skin calming antioxidant. Skin Barrier Protectors (Hydrators) Hyaluronic Acid Hyaluronic Acid is a humectant, meaning that it attracts water from the air or within the skin. Hyaluaronic Acid is one of the most common known ingredients for increasing overall moisture in skin. Vitamin C [Ascorbic Acid] Ascorbic Acid is an antioxidant and the most stable Vitamin C form. Ascorbic Acid aids in brightening the skin and aids in the production of collagen to prevent premature aging. Prebiotics Fructooligosaccharides Fructooligosaccharides is a prebiotic sugar used as food by the skin microbiota. It’s also a good source of antioxidants. It helps to keep the skin clean, hydrated, and well nutrified. It helps to maintain a healthy microbiome for your skin. Niacinamide Niacinamide is a vitamin that is water-soluble and penetrate into the skin with ease. Niacinamide aids in reducing the visibility of pores and removes dullness and restores the skin. Licorice Root Licorice Root is a plant based ingredients that can be cultivated in Asia, Europe, and in the Middle East. Licorice Root is a brightening agent in skincare products and can repair the skin. Lactic Acid Lactic Acid is a metabolic by-product released by the fermentation of microorganisms like lactobacillus. Lactic acid strengthens the skin barrier and has a positive impact on the skin microbiome balance. It also helps in reducing uneven tone and brightens skin tone.

Introducing the Biome Mask™ Sequential Skin is proud to announce its biodegradable Biome Mask™ line designed to balance the skin microbiome and make your skin healthier. The Biome Mask™ line has five formulas to target five skin traits. Each is infused with serums that target specific concerns and uses prebiotics and postbiotics to balance the skin's microbiome. All masks have been tested in vivo. Made for all skin types Our formula, your benefits Semi-gel Tencel™ Versatile, soft & pleasant to the skin Eco-friendly Material Better for you and the planet 100% Cellulose Cellulose is the most abundant natural organic compound on Earth Remarkable Moisture Retention For long lasting benefits Click Here to see a Full List of our Ingredients How They Work Prebiotics Prebiotics beneficially affect the host by selectively stimulating the growth and/or activity of one or a limited number of bacteria on the skin, and thus improves host health. Feed your skin microbiome! Prebiotics act as good for the microbes on the skin, and allow good bacteria to grow and thrive. Probiotics Probiotics are living microorganisms that confer benefit to the host when applied to the body. Because probiotics are live bacteria, it is incredibly difficult to preserve and put into skincare products (especially when skincare contains preservatives). In fact, some products on the market that say they use probiotics, are actually using prebiotics or postbiotics. Postbiotics Postbiotics are the byproduct of fermented live bacteria (probiotics) or inactivated microorganisms. The process of fermentation releases nutrients inside of the bacteria and holds concentrated and nourishing skin health benefits that help to balance the skin microbiome. INGREDIENTS FROM MICROBIAL ORIGIN No INTENDED TO BE UTILISED BY THE HUMAN MICROBIOTA? No Yes Yes ARE THEY VIABLE? No Yes ORIGIN VIABILITY FUNCTION ORDINARY INGREDIENT ORDINARY INGREDIENT WITH PREBIOTIC FUNCTION INTENDED TO BE UTILISED BY THE HUMAN MICROBIOTA? PROBIOTIC INGREDIENT No Yes POSTBIOTIC INGREDIENT POSTBIOTIC INGREDIENT WITH PREBIOTIC FUNCTION Also available in a box of 5 Biome Masks™ Interested? What is Sequential's testing platform? Sequential has developed the gold standard test for microbiome-friendly products, in vivo (in, or on, humans). Finally, we can give some certainty about if a product is truly affecting the microbiome. We offer a complete end-to-end solution to support microbiome-friendly claims. From consultancy and study design to our proprietary microbiome testing kits. We analyse, interpret and report our findings to meet your needs. Why is it necessary to test the microbiome in vivo? At present, there are no regulations for microbiome-related formulas that brands and formulators can follow, however, it has been universally acknowledged that the in vivo method of conducting clinical studies is becoming critical and paramount to getting marketing claims through. When regulations are introduced, which may be imminent, the in vitro system will find itself lacking, resulting in limited claims and certifications that do not hold their value. This is why, we at Sequential strive to offer an in vivo approach, knowing full well that we want our client's claims to be significantly backed by scientific and quantifiable data. What type of sequencing technology does Sequential use for analysis? We offer four types of sequencing techniques including qPCR with our Smart Probes™, 16S, ITS and Shotgun Metagenomics. Using next-generation sequencing of the collection of microorganisms found on the body, during product usage, Sequential investigates the microbial diversity, and particular microorganisms we know are important and play a role in a healthy microbiome. Does Sequential offer claims certification for tested products? We provide our clients with a certification to claim “Maintains the Microbiome” subject to in vivo testing results which can be used in communication efforts. Once your product is tested with our qPCR Smart Probes™ and has shown favourable results in supporting the microbiome, we can certify your product with our Maintains the Microbiome certification seal. We have ensured that our seal and certification are backed by quantifiable data and scientifically significant markers. The aim is to ensure our clients feel confident in making their claims and can communicate the true benefit of their microbiome formulations.

TARGETED SKIN PANEL Atopic Dermatitis Targeted Panel 23 Smart Probes ™ Atopic Dermatitis Panel Validated targets: Staphylococcus aureus, Malassezia spp, Staphylococcus epidermidis Absolute Quantification End-to-end Service Qet a Quote Atopic Dermatitis Study with Sequential's Cutting-Edge Microbiome Testing Why Microbiome Testing for Atopic Dermatitis? Atopic dermatitis (AD), a chronic inflammatory skin condition, affects around 31.6 million people in the U.S., with 15-20% of children globally and 7.3% of U.S. adults experiencing the condition (National Eczema Association). AD often results from a combination of genetic predisposition and environmental triggers, such as irritants in cosmetic products. Disruptions to the skin’s microbiome play a significant role in the disease's development and severity. Imbalances in beneficial and harmful microbes can exacerbate AD, highlighting the importance of targeted treatments. In vivo testing for products aimed at managing AD is essential because it allows researchers to understand how formulations impact both the skin barrier and microbiome in real-world conditions. Properly tested products can help reduce irritation and avoid worsening symptoms, offering a science-backed approach to skincare for those with AD. Sequential is leading the way in microbiome testing for the industry. With a specialized focus on skin conditions, we have developed an innovative solution tailored to help you understand the root causes of AD at the microbial level. Our proprietary sequencing panel is designed specifically to offer in-depth analysis of AD-associated microbiota, giving you the tools to transform skincare solutions. Customized Analysis Tailored insights specific to AD-prone skin, allowing your R&D team to develop more personalized and effective products. Actionable Data Leverage detailed reports to guide formulation decisions and validate product efficacy with precision. Product Innovation New AD treatments or enhancing existing lines, our data helps you create targeted solutions with proven results. Precision Sequencing Target specific microbial communities associated with AD development and severity. Smart Probe s ™ Our dedicated team of scientists has developed a revolutionary method for evaluating microbes through our Smart Probes™. This proprietary panel includes 23 key microbes that we have specifically identified as having the most significant impact on skin health. To further understand the intricate relationship between atopic dermatitis (AD) and the skin microbiome, we use qPCR technology. This advanced technique allows us to precisely quantify microbial populations on the skin, providing deeper insights into how AD affects the microbiome. By closely monitoring how products interact with both beneficial and harmful microbes, companies can create solutions for AD but also restore a healthy balance to the skin’s ecosystem. Atopic Dermatitis Panel Targets Staphylococcus aureus This bacterium is frequently found in higher numbers on the skin of individuals with atopic dermatitis. It can exacerbate inflammation and contribute to skin barrier disruption. Malassezia spp This genus of yeast, which is part of the normal skin flora, can become overgrown in individuals with atopic dermatitis, potentially aggravating symptoms. Staphylococcus epidermidis While generally considered a commensal organism, imbalances in its population, along with other microbes, can influence the skin's health and contribute to atopic dermatitis symptoms. Industry Leader in Absolute Quantification Sequential’s Smart Probe ™ qPCR approach to microbiome analysis can resolve species and subspecies, giving statistically significant findings between skin conditions on in vivo human samples, compared to conventional sequencing approaches. Relevant Research AOB for AD: Ammonium-oxidising Bacteria as an Innovative Approach to Managing Atopic Dermatitis Answers for Atopic Dermatitis and Allergies: What is the Role of the Skin Bacteriome? Understanding Atopic Dermatitis Read More Articles

Scalp Microbiome Testing As the scalp care industry grows, consumers demand transparency from the brands formulating their products. It has become essential for formulators to create products they are willing to test to present scientifically backed data-driven evidence of their products' true effects. Sequential offers microbiome testing for your scalp care formulations, ranging from scalp serums, shampoos, conditioners, oils, etc. We are dedicated to understanding how your product interacts with the scalp and its microbes. Depending on how in-depth you want to go, we offer qPCR, 16S, ITS, and Shotgun Metagenomics. Download Case Study! Personalized Approach to Testing Unlike other methodologies present within the industry, Sequential's approach ensures that your product's data and analysis will stand the test of industry regulations when they are introduced. You can tailor your study entirely to your unique requirements. Test Products in a Real-Life Context The microbiome comprises a complex ecosystem of microorganisms that live together in a delicate balance, which is why it's best to test directly upon it directly. To fully understand the impact a product is having on the microbiome, in vivo is the only way. Collect Longitudinal Data With in vivo testing we can design your study around the extended use of a product over multiple time points. This allows us to review how a product is performing before and after usage, but also take into account its gradual impact on the microbiome. Measure Against a Control Group Measure a product against a control group that might have a different percentage of your active ingredient within its formulation or no active at all. This will allow for deeper insights into the impact of a formulation on microbial balance and diversity. 4 Sequencing Reports To Pick From Depending on your development stage and what you are interested in studying we offer qPCR, 16S, ITS, and Shotgun Metagenomics. With our qPCR Smart Probes™ we can go down to the strain level in our analysis. Personalize Your Microbiome Study! Unlike hair, the scalp is formed of a living community of microorganisms such as bacterial and fungal players that can influence the balance of the scalp microbiome, even the subtlest of imbalances can lead to issues such as dandruff, itching, and irritation, resulting in unwanted flaking. Smart Probe s ™ Our dedicated team of scientists have developed a method of evaluating microbes through our Smart Probes ™ . These refer to a panel of 20 key microbes we have specifically identified as having the most impact on scalp health. Over and above the taxonomic characterisation that 16S offers, which gives us a snapshot of all the genus present within a collected sample (Cutibacterium , Staphylococcus , etc.) our targeted approach takes it a step further, opening the lens to the species (C. acnes ), sub-species (C. acnes defendens ) and even strains within them. This is a crucial distinction as not all strains of a species behave similarly. We find that within these species there are strains associated with inflammation and strains that are commensal, and beneficial. Gold Standard Certification Sequential has developed the gold standard test for products designed to target the microbiome, in vivo (in, or on, humans). Finally, we can give some certainty about if a product is truly affecting the microbiome. Using next-generation sequencing of the collection of micro-organisms found on the body, before and after product usage, Sequential investigates the microbial balance and diversity, and particular micro-organisms we know are important and play a role in a healthy microbiome. We give you an in vivo certification that your product maintains the microbiome. And it’s not exclusive to skincare! We do this for haircare products, oral products, and vulva/vaginal microbiomes. Personalize Your Microbiome Study! Supplement Your Microbiome Study Recruitment Services Let us take care of the entire candidate recruitment process for you! View More Biophysical Assessments Increase your data on the use of your product by evaluating additional biophysical factors. View More Formulation Support Seek consultation advice for your formulation if you are re-formulating or developing a new product. View More FAQ What is Sequential's testing platform? Sequential has developed the gold standard test for microbiome-friendly products, in vivo (in, or on, humans). Finally, we can give some certainty about if a product is truly affecting the microbiome. We offer a complete end-to-end solution to support microbiome-friendly claims. From consultancy and study design to our proprietary microbiome testing kits. We analyse, interpret and report our findings to meet your needs. Why is it necessary to test the microbiome in vivo? At present, there are no regulations for microbiome-related formulas that brands and formulators can follow, however, it has been universally acknowledged that the in vivo method of conducting clinical studies is becoming critical and paramount to getting marketing claims through. When regulations are introduced, which may be imminent, the in vitro system will find itself lacking, resulting in limited claims and certifications that do not hold their value. This is why, we at Sequential strive to offer an in vivo approach, knowing full well that we want our client's claims to be significantly backed by scientific and quantifiable data. What type of sequencing technology does Sequential use for analysis? We offer four types of sequencing techniques including qPCR with our Smart Probes™, 16S, ITS and Shotgun Metagenomics. Using next-generation sequencing of the collection of microorganisms found on the body, during product usage, Sequential investigates the microbial diversity, and particular microorganisms we know are important and play a role in a healthy microbiome. Does Sequential offer claims certification for tested products? We provide our clients with a certification to claim “Maintains the Microbiome” subject to in vivo testing results which can be used in communication efforts. Once your product is tested with our qPCR Smart Probes™ and has shown favourable results in supporting the microbiome, we can certify your product with our Maintains the Microbiome certification seal. We have ensured that our seal and certification are backed by quantifiable data and scientifically significant markers. The aim is to ensure our clients feel confident in making their claims and can communicate the true benefit of their microbiome formulations.